Vibrio Cholerae, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1839

This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.

Preface…

Table of Contents…

Contributing Authors…

1. Laboratory Culturing Techniques and Maintenance of Vibrio cholera

Kyle D. Brumfield, Bailey M. Carignan, and Mike S. Son

2. Genotypic and Phenotypic Assays to Distinguish Vibrio cholera Biotype

Kyle D. Brumfield, Bailey M. Carignan, and Mike S. Son

3. Preparation of Vibrio cholera Samples for RNA-seq Analysis

Jyl S. Matson

4. Random Transposon Mutagenesis of Vibrio cholera

Jyl S. Matson

5. Metabolomics of Vibrio cholera

Yusuke Minato, Jay S. Kirkwood, and Claudia C. Häse

6. Natural Cotransformation and Multiplxed Genome Editing by Natural Transformation (MuGENT) of Vibrio cholera

Ankur B. Dalia

7. Vibrio cholera Chromatin Immunoprecipitation

Julio C. Ayala, Jorge A. Benitez, and Anisia J. Silva

8. Fly Models of Vibrio cholera Infection and Colonization

Alexandra E. Purdy

9. Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholera

Kristie C. Mitchell and Jeffrey H. Withey

10. Transposon Sequencing of Vibrio cholera in the Infant Rabbit Model of Cholera

Lauren M Shull and Andrew Camilli

11. Isolation of Outer Membrane Vesicles Including their Quantitative and Qualitative Analyses

Paul Kohl, Franz G. Zingl, Tom Eichmann, and Stefan Schild

12. Utilization of Vibrio cholera as a Model Organism to Screen natural Product Libraries for Identification of New Antibodies

Aleksandra E. Sikora, Richard Tehan, and Kerry PcPhail

13. Infant Mouse Model of Vibrio cholera Infection and Colonization

Jyl S. Matson

Bo R. Weber and Aleksandra E. Sikora

15. Proteomics of Vibrio cholerarea

Ryszard A. Zielke

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts