Protocols and Applications in Enzymology
Auteurs : Belorkar Seema Anil, Jogaiah Sudisha
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.
1. Introduction Enzymes-Past, present and Future 2. Distribution and diversity in microbial enzymes 3. Screening of potential microbes for enzymes of industrial significance 4. Solid state fermentations and SMF for enzyme production 5. Laboratory methods for enzyme assays 6. Structure and functions of enzyme kinetics 7. Protocols of important industrial enzymes 8. Strategies to improve enzyme activity for industrial processes 9. Scope and relevance of Industrial applications 10. Agro-industrial wastes for enzyme production 11. Significance of enzymes and their agricultural applications 12. Biotechnological applications of enzymes and future prospective 13. Conclusions
Dr. Sudisha Jogaiah, Associate Professor and Head, Department of Environmental Science, Central University of Kerala. He is the Fellow of the National Academy of Agricultural Sciences (FNAAS), Fellow of National Academy of Biological Sciences (FNABS), Bridge Fellow, Government of Japan. He is the recipient of Sir C.V. Raman Young Scientist State Award by Chief Minister of Karnataka. He was awarded visiting Professorship by Yamaguchi University, Japan and Nanjing Agricultural University, China. He joined as Associate Professor in the Department of Environmental Science, Central University of Kerala in 2022. The technological quality and expertise of Dr. Jogaiah’s lab is reflected in an extensive track record of publications with over 100 peer-reviewed papers with cumulative Thomson Reuters impact factor of 461.9, citations above 5053, one national and one International patent and 07 review articles with h-index - 41.
- Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards
- Presents easy to read, reproducible protocols for researchers and students across academia and industry
- Includes color diagrams that illustrate key concepts
Date de parution : 11-2021
Ouvrage de 266 p.
19x23.4 cm
Mots-clés :
Achievements and upcoming possibilities of enzymes usage; Agroindustrial wastes; Applications of enzymes in different walks of life and future applications; Assay; Biochemical reactions they cater; Biopesticides and biomicrobicides; Cellulases; Conclusion; Conventional screening techniques; Enzyme engineering; Enzyme kinetics; Enzymes; Enzymes active in industries; Enzymology; Feasibility and economic viability; Fructosyltransferases; Functions and structures; Fundamental discoveries; Future prospectives; History; Immobilization of enzyme; Increasing fertility; Industrially important organisms; Lipases and proteases; Major classes of enzymes; Market demand of enzymes and membrane bioreactors; Medium engineering; Methodologies of SSF and SMF with their potential benefits; Nature and its role in extremophilic enzymes; Parameters; Productivity; Proteeases and xylanases; Protocols; Recycling; Strategy of enzyme usage; Substrate engineering; Xylanases
