Practical fluorescence microscopy in mammalian cells: protein localization and function

This volume presents a multidisciplinary approach to microscopy emphasizing a thorough and integrative approach to imaging experiments starting with a testable hypothesis (question), proceeding through the test-tube stage of making the reagents to the microscopy stage of introducing the reagents into the cells and their eventual imaging. Each chapter culminates with the advice on the interpretation and analysis of images to determine your experimental results. This book addresses the practical questions regarding benefits and pitfalls of various techniques and the interpretation of results. Each technique is illustrated by photomicrographs showing the "normal" appearance of the structure or phenomenon studied as well as methodology- related artifacts.