Notch Signaling Research, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2472

1. Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster

            Oleg V. Andreyenkov, Elena I. Volkova, Natalya G. Andreyenkova, and Sergey A. Demakov

2. Screening Mutants by Single Fly Genomic PCR

            Usha Nagarajan and Marios Georgiou

3. Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation

            Chenyu Ma, Yohei Tsukamoto, and Hideyuki Takeuchi

4. Use of FLP/FRT System to Screen for Notch Signaling Regulators in the DrosophilaWing

5. High-Throughput Analysis to Identify Activators of Notch Signaling

            Rachael Guenter, Jacob Eide, Herbert Chen, J. Bart Rose, and Renata Jaskula-Sztul

6. Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads

            Takamasa Mizoguchi, Hikaru Handa, Shuhei Omaru, and Motoyuki Itoh

7. Mammalian NOTCH Receptors Activation and Signaling Protocols

            María Luisa Nueda and Victoriano Baladrón

8. Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation

            Vartika Sharma, Nalani Sachan, Mousumi Mutsuddi, and Ashim Mukherjee

9. Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit

            Kostiantyn Dreval, Robert J. Lake, and Hua-Ying Fan

10. Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters

            Manoj Panta, Andrew J. Kump, Kristopher R. Schwab, and Shaad M. Ahmad

11. Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context

            ChangHwan Lee, Tina Lynch, Sarah L. Crittenden, and Judith Kimble

12. An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers

            Lily Paculis, Qiuping Xu, Qian Xie, and Dongyu Jia

13. Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster

            Cordula Schulz

14. Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos

            Elisabete Silva, Patrícia Diniz, Alexandre Trindade, Mariana Batista, Ana Torres, António Duarte, and Luís Lopes-da-Costa

15. Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies

            Laura Márquez-Expósito, Carolina Lavoz, Elena Cantero-Navarro, Raúl R. Rodrigues-Diez, Sergio Mezzano, and Marta Ruiz-Ortega

16. Exosomes as Carriers for Notch Molecules

            Guya Diletta Marconi, Francesca Diomede, Oriana Trubiani, Cristina Porcheri, and Thimios A. Mitsiadis

17. In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease

            Pritesh P. Jain, Susumu Hosokawa, Aleksandra Babicheva, Tengteng Zhao, Jiyuan Chen, Patricia A. Thistlethwaite, Ayako Makino, and Jason X.-J. Yuan

18. Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer

            Shiqin Liu, En-chi Hsu, Michelle Shen, Merve Aslan, and Tanya Stoyanova

19. Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila

            Sheng-An Yang, Jose L. Salazar, David Li-Kroeger, and Shinya Yamamoto

20. Bioinformatics Tools to Understand Notch

            Ashley Avila, Roxana Gonzalez Tascon, and Dongyu Jia

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts