Pain research: methods and protocols (Methods in molecular medicine, Vol. 99)
Langue : Anglais
Auteur : LUO Z david
Noted scientists provide step-by-step methods and protocols for the study of pain transduction. They cover everything from animal models resembling the pathological conditions of pain transduction, and protocols to understand the interactions among pain transducers and mediators at the molecular level. The authors present a variety of multidisciplinary approaches to dissecting the molecular mechanisms of pain transduction making this essential for all pain researchers.
Mechanistic Dissection of Pain: From DNA to Animal Models. Assessment of Acute Thermal Nociception in Laboratory Animals. Tissue Injury Models of Persistent Nociception in Rats Jeffrey. Segmental Spinal Nerve Ligation Model of Neuropathic Pain. Partial Sciatic Nerve Transection. Modeling Diabetic Sensory Neuropathy in Rats. Sciatic Inflammatory Neuropathy in the Rat: Surgical Procedures, Induction of Inflammation, and Behavioral Testing. A Rat Pain Model of Vincristine-Induced Neuropathy. Loop Dialysis Catheter: A Technology for Chronic Spinal Dialysis in A Freely Moving Rat. Intrathecal Catheterization and Drug Delivery in the Rat Shelle. Trigeminal Neuronal Recording in Animal Models of Orofacial Pain. In Vivo Electrophysiology of Dorsal Horn Neurons. Single Fiber Recording: In Vivo and In Vitro Preparations. Anatomical Identification of Neurons Responsive to Nociceptive Stimuli. Isolation and Culture of Sensory Neurons from the Dorsal Root Ganglia of Embryonic or Adult Rats. Primary cultures of Neonatal Rat Spinal Cord. Single-Cell Laser Capture Microdissection and RNA Amplification. Semi-Quantitative Real-Time PCR for Pain Research. Functional Genomic Analysis in Pain Research Using Hybridization Arrays. Generation of Transgenic Mice. Knockout Mouse Models in Pain Research.
Date de parution : 06-2004
Ouvrage de 320 p.
15x23 cm
Thèmes de Pain research: methods and protocols ... :
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