Embryonic Stem Cells : Methods and Protocols (Methods in Molecular Biology vol. 185)

Kursad Turksen and a panel of international experts describe their most productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, the generation of antibodies against very rare antigens, and the identification of and characterization of protein and protein interactions. This volume will prove an invaluable resource not only for those interested generally in cell and developmental biology, but also for those actively using, or planning to use ES cells, to study fate choices and specific lineages.